How Is Endotoxin Tested with TAL Reagent

2021-03-17

Lyophilized Amebocyte Lysate assay (TAL/LAL assay) is the most commonly used test for endotoxin. Tachypleus Amebocyte Lysate (TAL), i.e. Limulus Amebocyte Lysate (LAL), is a kind of lyophilized product which mainly contains amoebocytes extracted from the blue blood of horseshoe crab.


There are mainly three techniques for this test: the gel clot technique, the turbidimetric technique, and the chromogenic technique. For gel clot method, it is based on gel formation. The turbidimetric method is based on forming turbidity after cleavage of an endogenous substrate, and chromogenic method is based on the producing color after cleavage of a synthetic peptide chromogen complex.


The greatest sensitivity, λ, of gel clot TAL produced by Xiamen Bioendo is 0.015EU/ml. It is 0.01EU/ml for end-point chromogenic TAL, 0.005EU/ml for kinetic turbidimetric TAL, and 0.001EU/ml for kinetic chromogenic TAL.

Regarding materials and equipment, only water bath or dry heat incubator which is able to maintaining 37±1is needed for gel clot technique when you use BIOENDO single test vial or ampoule. 10 x 75 mm fully depyrogenated borosilicate glass tubes (BIOENDO Endotoxin-free Glass Test Tubes, Catalog No.: T107530) are needed when you choose BIOENDO multi-test TAL vial. Optical reader is capable of reading at 405nm, or at 540-550nm are needed for turbidimetric technique and chromogenic technique. Others include test tube racks to hold the tubes and/or incubate dilution and reaction tubes, micro-pipettes or disposable pipette tips free of interfering endotoxins and glucans, vortex-type mixer.


About results, the gel clot technique shall test the integrity of the gel. A result is positive if a firm gel has formed and remains in place upon inversion; it is negative if an intact gel is not formed. For turbidimetric technique and chromogenic technique, BIOENDO Kinetic Incubating Microplate Reader ELx808IULALXH with relevant software will read and process related data.

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