How To Use Limulus Reagent Correctly

2019-06-28

The accuracy of endotoxin test results is closely related to the sensitivity of tachypleus amebocyte lysate reagent, and the sensitivity of tachypleus amebocyte lysate reagent is closely related to its correct use. Therefore, we must strictly enforce the quality standard of limulus reagent and standardize the use of limulus reagent. The quality of limulus lysate reagents is mainly shown in three aspects: sensitivity, self coagulation time and gelation state: sensitivity is stable; the self setting time is not less than 24h; it has certain buffering capacity and suitable pH; after reaction, a solid gel is formed, and the plastic ampoule is turned 180 degrees without being damaged.

However, the sensitivity of limulus reagent with slightly poor quality may change after storage for a certain period of time (for example, half a year), and the change of sensitivity may change the allowable limit of endotoxin in the test product reflected by the determination results.

Therefore, it is necessary to choose the limulus reagent with stable quality approved by the state. Because the quality of tachypleus amebocyte lysate reagents from different manufacturers is different, even the tachypleus amebocyte lysate reagents from different batches from the same manufacturer are different, which leads to the difference between the sensitivity of tachypleus amebocyte lysate reagents and the labeled value. Therefore, the reagents have a significant impact on the test results. Sensitivity should be checked every time a new batch of tachypleus amebocyte lysate reagent is used and every six months from the date of production the reagent is retained. The sensitivity should meet the requirements within the validity period.

Some studies have shown that the sensitivity of tachypleus amebocyte lysate reagent has little change and is stable within 3 years under the condition of meeting the standard and proper storage. It is suggested that the period of validity should be extended. Therefore, monitoring the sensitivity of tachypleus amebocyte lysate reagent is helpful to ensure the accuracy of the experiment, and can be used to the greatest extent.

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