microKC Kinetic Chromogenic LAL (Micro Kinetic Chromogenic Assay) is a miniaturized kinetic chromogenic test that uses low-volume 96-well microplates/strips, compatible with the ELx808IU-SN LAL system and other standard endotoxin detection instruments. It fully meets pharmacopoeial requirements for bacterial endotoxin testing, requiring only 25 µL of sample and 25 µL of LAL reagent per test. Sharing the same detection principle as the traditional kinetic chromogenic method, it offers strong anti-interference capability and is ideally suited for quantitative endotoxin detection in injectable drugs, vaccines, antibodies, biologics, and other pharmaceutical products.
| Catalog No. | Description & Kit Contents | Sensitivity Range |
| MKC0505 | 90 Tests/Kit 5 Chromogenic Amebocyte Lysate; 5 Reconstitution Buffer; 3 CSE; 2 Water for BET | 0.005-10 EU/mL |
MPMC96 （sold separately） | Microplate strip, 8 Wells/Strip, 12 pcs/Pack 96 wells per microplate, 12pcs detachable strips |
The sensitivity of Lyophilized Amebocyte Lysate and potency of Control Standard Endotoxin are assayed against USP Reference Standard Endotoxin. The Lyophilized Amebocyte Lysate reagent kits come with product instruction, Certificate of Analysis, MSDS.
Note: For other sizes/specifications not listed above, please contact our customer service team.
Email: Eve@bioendo.com
Tel: +86 18065928551
ANTI-INTERFERENCE
Unique Formulation
Unique formulation effectively copes with complex matrix interference.
COST-EFFECTIVE
25 µL per test
Uses only 25 µL per test for both sample and LAL reagent, significantly reducing experimental costs.
WIDE RANGE
0.005–10 EU/mL
Detection range of 0.005–10 EU/mL, much broader than the gel clot method, allowing more flexible dilution and facilitating the establishment of suitable endotoxin detection methods.
TREND ASSESSMENT
Recovery Rate Interference Identification
Enables interference trend identification through recovery rates, particularly advantageous for method development with R&D-stage samples such as new products.
EASY OPERATION
Vial Format & Automated Compatibility
Supplied in vials for convenient pipetting; compatible with automated absorbance and fluorescence detection instruments for one-step complete analysis.
RELATED EQUIPMENT
Compatible Endotoxin Detection Systems
Compatible instruments support different endotoxin detection workflows, from routine laboratory testing to fully automated analysis.
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FREQUENTLY ASKED QUESTIONS
Micro Kinetic Chromogenic Assay
Q1
What types of samples are suitable for the Micro Kinetic Chromogenic Assay?
Samples that are numerous, limited in volume, or require high-sensitivity reagents.
Q2
Is the reconstitution buffer provided with the LAL reagent for the Micro Kinetic Chromogenic Assay?
Yes, they are compatible and provided as a matched set.
Q3
What instruments are required for the Micro Kinetic Chromogenic Assay, and what are the instrument specifications?
A microplate reader or a rapid microbial detection system for LAL testing is required. The instrument must be equipped with a 405 nm detection wavelength, a temperature control system, and kinetic analysis capabilities.
Q4
Can reagents from different kit lots be mixed in the Micro Kinetic Chromogenic Assay?
Mixing reagents from different lots is not recommended.
Q5
Are consumables provided for the Micro Kinetic Chromogenic Assay?
Yes, consumables are available upon request.
Q6
What is the difference between the Micro Kinetic Chromogenic Assay and the conventional Kinetic Chromogenic Assay?
The Micro Kinetic Chromogenic Assay offers higher sensitivity, lower reagent consumption, and a broader linear range compared to the conventional Kinetic Chromogenic Assay.
Q7
What is the sensitivity of the Micro Kinetic Chromogenic Assay?
The sensitivity ranges from 5 to 0.005 EU/ml, and can reach up to 50 to 0.001 EU/ml under optimized conditions.